Facile barometric calibration of photoluminescence-based oxygen sensors on a standard rotary evaporator system with a digital vacuum pump

• DOI: 10.1016/j.snb.2024.136286
• Publication Date: 2024-07-10T18:17:10Z
• Authors: Liang Li; Rafael D.L. Gaspar; Dmitri B. Papkovsky
• Keywords: Vacuum pump

A simple setup and methodology for barometric calibration of quenched phosphorescence oxygen sensors are described, which utilize a standard rotary evaporator (Rotavap) system equipped with digital vacuum pump. They were demonstrated panel different sensing materials based on PtBP dye, including solid-state sensor coatings substrates (membrane inserts, stickers or inner wall the flask), as well liquid formulations. The inside flask exposed to conditions created by Rotavap (dry humid air, water, temperatures ranging 2°C-40°C, residual total pressure), their intensity, lifetime phase shift signals measured handheld readers Optech (Mocon) Firesting (PyroScience) in contact-less manner generate multi-point curves. Several critical points method, require careful consideration, have been revealed, mounting Rotavap, its temperature control during calibration, choice reader. Finally, detailed characterization several types was carried out key operational parameters (τ0, KSV, heterogeneity, T-coefficients), equations determined compared each other results conventional O2 calibration. Overall, method using provides useful alternative methods, being simple, accurate, convenient versatile.