Visualization of individual cell division history in complex tissues using iCOUNT

• DOI: 10.1016/j.stem.2021.08.012
• Publication Date: 2021-09-14T14:31:29Z
• Authors: Annina Denoth-Lip...; Baptiste N. Jaeger; Tong Liang; Lars N. Royall; Stefanie E. Chie; Kilian Buthey; Diana Machado; Vladislav I. Koro...; Merit Kruse; Clara M. Munz; Alexander Gerbaulet; Benjamin D. Simons; Sebastian Jessberger
• Keywords: Forebrain; Developmental Biology

The division potential of individual stem cells and the molecular consequences successive rounds proliferation remain largely unknown. Here, we developed an inducible cell counter (iCOUNT) that reports events in human mouse tissues vitro vivo. Analyzing histories neural stem/progenitor (NSPCs) developing adult brain, show iCOUNT can provide novel insights into behavior. Further, use single-cell RNA sequencing (scRNA-seq) iCOUNT-labeled NSPCs their progenies from cortex forebrain-regionalized organoids to identify functionally relevant pathways are commonly regulated between cells, depending on histories. Thus, a tool characterize repeated divisions allows analysis cellular principles underlying tissue formation, homeostasis, repair.