Generation of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells

• DOI: 10.1016/j.stemcr.2014.07.005
• Publication Date: 2014-08-21T15:47:45Z
• Authors: Shimpei Gotoh; Isao Ito; Tadao Nagasaki; Yuki Yamamoto; Satoshi Konishi; Yohei Korogi; Hisako Matsumoto; Shigeo Muro; Toyohiro Hirai; Michinori Funato; Shin-Ichi Mae; Taro Toyoda; Aiko Sato-Otsubo; Seishi Ogawa; Kenji Osafune; Michiaki Mishima
• Keywords: Embryoid body; KOSR; Amniotic epithelial cells

No methods for isolating induced alveolar epithelial progenitor cells (AEPCs) from human embryonic stem (hESCs) and pluripotent (hiPSCs) have been reported.Based on a study of the stepwise induction (AECs), we identified carboxypeptidase M (CPM) as surface marker NKX2-1 + ''ventralized'' anterior foregut endoderm (VAFECs) in vitro fetal murine lungs.Using SFTPC-GFP reporter hPSCs 3D coculture system with lung fibroblasts, showed that CPM isolated VAFECs differentiate into AECs, demonstrating is AEPCs.Moreover, differentiation formed spheroids lamellar-body-like structures an increased expression surfactant proteins compared 2D differentiation.Methods to induce isolate AEPCs using consequently generate would aid pulmonary disease modeling regenerative medicine.