Pten Cell Autonomously Modulates the Hematopoietic Stem Cell Response to Inflammatory Cytokines

• DOI: 10.1016/j.stemcr.2016.04.008
• Publication Date: 2016-05-12T18:15:05Z
• Authors: Shaina N. Porter; Andrew S. Cluster; Robert A.J. Signer; Jenna Voigtmann; Darlene A. Monlish; Laura G. Schuettpelz; Jeffrey A. Magee
• Keywords: Hematopoietic stem cell

Pten negatively regulates the phosphatidylinositol 3-kinase (PI3K) pathway and is required to maintain quiescent adult hematopoietic stem cells (HSCs). has been proposed regulate HSCs cell autonomously non-cell autonomously, but relative importance of each mechanism not directly tested. Furthermore, cytokines that activate PI3K upstream are well defined. We sought clarify whether or HSC mobilization. also tested deficiency affects response granulocyte colony-stimulating factor (G-CSF) interferon-α (IFNα) since these induce mobilization proliferation, respectively. show expansion in spleen primarily via cell-autonomous mechanisms. Pten-deficient do require G-CSF mobilize, although they hyper-sensitized even low doses exogenous G-CSF. similarly sensitized IFNα. therefore modulates inflammatory cytokines.