Primary Human Testicular Cells Self-Organize into Organoids with Testicular Properties

• DOI: 10.1016/j.stemcr.2016.11.012
• Publication Date: 2016-12-23T02:45:30Z
• Authors: Yoni Baert; Joery De Kock; João P. Alves-Lopes; Olle Söder; Jan-Bernd Stukenborg; Ellen Goossens
• Keywords: Organoid

So far, successful de novo formation of testicular tissue followed by complete spermatogenesis in vitro has been achieved only rodents. Our findings reveal that primary human cells are able to self-organize into organoids (TOs), i.e., multi-cellular surrogates, either with or without support a biological scaffold. Despite lacking testis-specific topography, these mini-tissues harbored spermatogonia and their important niche cells, which retained specific functionalities during long-term culture. These observations indicate the posibility re-engineering microenvironment from cells. Human TOs might help development biomimetic model would exert tremendous impact on research development, clinical treatment infertility, screening connection drug discovery toxicology.