SCA-1 Labels a Subset of Estrogen-Responsive Bipotential Repopulating Cells within the CD24 + CD49f hi Mammary Stem Cell-Enriched Compartment

• DOI: 10.1016/j.stemcr.2016.12.022
• Publication Date: 2017-01-26T12:48:41Z
• Authors: Genevieve V. Dall; Jessica L. Vieusseux; Kenneth S. Korach; Yukitomo Arao; Sylvia C. Hewitt; Katherine J. Hami...; Elaine Dzierzak; Wah Chin Boon; Evan R. Simpson; Robert G. Ramsay; Torsten Stein; Joanne S. Morris; Robin L. Anderson; Gail P. Risbridger; Kara L. Britt
• Keywords: CD24; Estrogen receptor beta

Estrogen stimulates breast development during puberty and mammary tumors in adulthood through estrogen receptor-α (ERα). These effects are proposed to occur via ERα+ luminal cells not the stem (MaSCs) that ERαneg. Since express cell antigen-1 (SCA-1), we sought determine if SCA-1 could define an subset of EpCAM+/CD24+/CD49fhi MaSCs. We show MaSC population has a distinct SCA-1+ is abundant pre-pubertal glands. The MaSCs have less markers vivo repopulating activity than their SCA-1neg counterparts. However, they ERα specifically enter cycle at puberty. Using estrogen-deficient aromatase knockouts (ArKO), showed be directly modulated by supplementation. Thus, enriches for ERα+, estrogen-sensitive subpopulation within CD24+/CD49fhi may responsible hormonal sensitivity developing gland.