Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models

• DOI: 10.1016/j.stemcr.2017.10.003
• Publication Date: 2017-11-05T23:44:11Z
• Authors: Sunao Sugita; Kenichi Makabe; Shota Fujii; Yuko Iwasaki; Hiroyuki Kamao; Takashi Shiina; Kazumasa Ogasawara; Masayo Takahashi
• Keywords: Graft Rejection; 0301 basic medicine; Medicine (General); Antigen Presentation; B-Lymphocytes; QH301-705.5; T-Lymphocytes; Induced Pluripotent Stem Cells; Cell Differentiation; Retinal Pigment Epithelium; Article; 3. Good health; Macaca fascicularis; 03 medical and health sciences; R5-920; Isoantibodies; Animals; Transplantation, Homologous; Biology (General); Cells, Cultured; Stem Cell Transplantation

Antibody-mediated rejection is characterized by donor-specific antibody produced B cells. However, to our knowledge, cell invasion and in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE could be performed using allografts, we established vivo immune models with induced pluripotent stem (iPSC)-RPE allografts determined whether RPE-specific detected these models. We alloantibodies serum from recipient monkeys that had attacks an immunofluorescent assay transplanted iPSC-RPE as antigen. In addition T antigen-presenting immunity, peripheral blood lymph nodes animal allogeneic also activated cells, which were probably secreting alloantibodies. Using alloreactive can for diagnosis transplantation.