Induction of Mesoderm and Neural Crest-Derived Pericytes from Human Pluripotent Stem Cells to Study Blood-Brain Barrier Interactions

• DOI: 10.1016/j.stemcr.2019.01.005
• Publication Date: 2019-02-10T16:03:03Z
• Authors: Tannaz Faal; Duc T.T. Phan; Hayk Davtyan; Vanessa M. Scarfone; Erika Varady; Mathew Blurton-Jones; Christopher C.W. ...; Matthew A. Inlay
• Keywords: Pluripotent Stem Cells; 0301 basic medicine; Aging; Medicine (General); Cerebrovascular; QH301-705.5; 1.1 Normal biological development and functioning; Clinical Sciences; Induced Pluripotent Stem Cells; 610; Neurodegenerative; Alzheimer's Disease; pericytes; Tight Junctions; Mesoderm; 03 medical and health sciences; R5-920; Stem Cell Research - Nonembryonic - Human; Alzheimer Disease; Report; mesoderm; Acquired Cognitive Impairment; 2.1 Biological and endogenous factors; Humans; human pluripotent stem cells; Stem Cell Research - Embryonic - Human; Biology (General); Stem Cell Research - Induced Pluripotent Stem Cell - Human; Stem Cell Research - Induced Pluripotent Stem Cell; Neurosciences; Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD); Brain; 600; Biological Sciences; Alzheimer's disease; blood-brain barrier; Stem Cell Research; endothelial cells; Brain Disorders; 3. Good health; Blood-Brain Barrier; Neural Crest; Biochemistry and cell biology; Neurological; Dementia; Biochemistry and Cell Biology; Pericytes; neural crest; Biomarkers

Highlights•Mesoderm and neural crest-derived pericytes (PCs) from hPSCs termed mPCs ncPCs•mPCs ncPCs express general brain-specific pericyte genes•mPCs associate with promote barrier properties of endothelial cells•WNT is important, but not necessary for PC specificationSummaryIn the CNS, perivascular cells ("pericytes") to mediate formation tight junctions essential function blood-brain (BBB). The BBB protects CNS by regulating flow nutrients toxins into out brain. dysfunction has been implicated in progression Alzheimer's disease (AD), role AD well understood. In developing embryo, originate two sources: mesoderm crest. this study, we report protocols using or crest intermediates, generate pericyte-like induced pluripotent stem cell (iPSC) lines created healthy patients. iPSC-derived display stable expression surface markers genes are functionally capable increasing vascular tube properties.Graphical abstract