An Extended Culture System that Supports Human Primordial Germ Cell-like Cell Survival and Initiation of DNA Methylation Erasure

• DOI: 10.1016/j.stemcr.2020.01.009
• Publication Date: 2020-02-13T15:33:42Z
• Authors: Joanna J. Gell; Wanlu Liu; Enrique Sosa; Alex Chialastri; Grace Hancock; Yu Tao; Sissy E. Wamaitha; Grace Bower; Siddharth S. Dey; Amander T. Clark
• Keywords: Reprogramming

The development of an in vitro system which human primordial germ cell-like cells (hPGCLCs) are generated from pluripotent stem (hPSCs) has been invaluable to further our understanding cell (hPGC) specification. However, the means evaluate next fundamental steps have not well established. In this study we describe a two dimensional extended culture that promotes proliferation specified hPGCLCs, without reversion state. We demonstrate hPGCLCs undergo partial epigenetic reprogramming, mirroring events described hPGCs vivo, including genome-wide reduction DNA methylation and maintenance depleted H3K9me2. This provides new approach for expanding number downstream technologies, transplantation, molecular screening, or possibly differentiation into gametes by gametogenesis.