Electrophysiological Maturation of Cerebral Organoids Correlates with Dynamic Morphological and Cellular Development

• DOI: 10.1016/j.stemcr.2020.08.017
• Publication Date: 2020-09-24T10:38:03Z
• Authors: Summer R. Fair; Dominic Julian; Annalisa M. Hartlaub; Sai Teja Pusuluri; Girik Malik; Taryn L. Summerfied; Guomao Zhao; Arelis B. Hester; William E. Ackerman; Ethan W. Hollings...; Mehboob Ali; Craig A. McElroy; Irina A. Buhimschi; Jaime Imitola; Nathalie L. Maitre; Tracy A. Bedrosian; Mark E. Hester
• Keywords: Organoid; Bursting; Brain Development; Human brain

Cerebral organoids (COs) are rapidly accelerating the rate of translational neuroscience based on their potential to model complex features developing human brain. Several studies have examined electrophysiological and neural network COs; however, no study has comprehensively investigated developmental trajectory properties in whole-brain COs correlated these with developmentally linked morphological cellular features. Here, we profiled neuroelectrical activities over span 5 months a multi-electrode array platform observed emergence maturation several electrophysiologic properties, including rapid firing rates bursting events. To complement analyses, characterized molecular development that gives rise mature immunohistochemical single-cell transcriptomic analyses. This integrated approach highlights value as an emerging system brain neurological disease.