Low Immunogenicity and Immunosuppressive Properties of Human ESC- and iPSC-Derived Retinas

• DOI: 10.1016/j.stemcr.2021.02.021
• Publication Date: 2021-03-25T14:37:09Z
• Authors: Suguru Yamasaki; Sunao Sugita; Matsuri Horiuchi; Tomohiro Masuda; Shota Fujii; Kenichi Makabe; Akihiro Kawasaki; Takuya Hayashi; Atsushi Kuwahara; Akiyoshi Kishino; Toru Kimura; Masayo Takahashi; Michiko Mandai
• Keywords: Immunosuppression Therapy; Primates; 0303 health sciences; Histocompatibility Antigens Class I; Human Embryonic Stem Cells; Induced Pluripotent Stem Cells; Cell Differentiation; Retinal Pigment Epithelium; Article; Recombinant Proteins; Retina; 3. Good health; Immunomodulation; Interferon-gamma; 03 medical and health sciences; Transforming Growth Factor beta; Leukocytes, Mononuclear; Animals; Humans

ESC- and iPSC-derived retinal transplantation is a promising therapeutic approach for disease with end-stage degeneration, such as retinitis pigmentosa age-related macular degeneration. We previously showed medium- to long-term survival, maturation, light response of transplanted human iPSC-retina in mouse, rat, monkey models Because the use patient hiPSC-derived retina disease-causing gene mutation not appropriate use, allogeneic using tissue/cells differentiated from stocked hESC iPSC line would be most practical. Here, we characterize immunological properties hESC- present their three major advantages: (1) expressed low levels leukocyte antigen (HLA) class I little HLA II vitro, (2) greatly suppressed immune activation lymphocytes co-culture, (3) activated cells partially via transforming growth factor β signaling. These results support future clinical application.