Hematopoietic stem cells retain functional potential and molecular identity in hibernation cultures

• DOI: 10.1016/j.stemcr.2021.04.002
• Publication Date: 2021-05-06T20:10:29Z
• Authors: Caroline A. Oedek...; Miriam Belmonte; Daniel Bode; Fiona K. Hamey; Mairi S. Shepherd; James Lok Chi Che; Grace Boyd; Craig McDonald; Serena Belluschi; Evangelia Diamanti; Hugo P. Bastos; Katherine S. Bridge; Berthold Göttgens; Elisa Laurenti; David G. Kent
• Keywords: Hibernation; Chimera (genetics); Hematopoietic stem cell

Advances in the isolation and gene expression profiling of single hematopoietic stem cells (HSCs) have permitted in-depth resolution their molecular program. However, long-term HSCs can only be isolated to near purity from adult mouse bone marrow, thereby precluding studies program different physiological states. Here, we describe a powerful 7-day HSC hibernation culture system that maintains as absence physical niche. Single hibernating retain full functional potential compared with freshly respect colony-forming capacity transplantation into primary secondary recipients. Comparison profiles counterparts showed striking degree similarity, further resolving core machinery self-renewal while also identifying key factors are potentially dispensable for function, including members AP1 complex (Jun, Fos, Ncor2), Sult1a1 Cish. Finally, provide evidence transduced without compromising activity demonstrate applicability cultures human HSCs.