A eutherian-specific microRNA controls the translation of Satb2 in a model of cortical differentiation

• DOI: 10.1016/j.stemcr.2021.04.020
• Publication Date: 2021-05-20T14:49:13Z
• Authors: Manuella Martins; Silvia Galfrè; Marco Terrigno; Luca Pandolfini; Irene Appolloni; Keagan Dunville; Andrea Marranci; Milena Rizzo; Alberto Mercatanti; Laura Poliseno; Francesco Morandin; Marco Pietrosanto; Manuela Helmer-Ci...; Paolo Malatesta; Robert Vignali; Federico Cremisi
• Keywords: 0301 basic medicine; 570; 571; in vitro corticogenesi; SATB2, cell fate, cell identity, corpus callosum, cortex, cortical layering, developmental timing, in vitro corticogenesis, mammalian evolution, miR-catch, microRNAs, neural stem cells, post-transcriptional control; Neurogenesis; 610; in  post-transcriptional control; Article; Cell Line; corpus callosum; neural stem cell; Mice; 03 medical and health sciences; SATB2; cell fate; cell identity; corpus callosum; cortex; cortical layering; developmental timing; in vitro corticogenesis; mammalian evolution; miR-catch; microRNA; neural stem cells; post-transcriptional control; cell fate; cell identity; corpus callosum; cortex; cortical layering; developmental timing; in vitro corticogenesis; mammalian evolution; microRNA; miR-catch; neural stem cells; post-transcriptional control; SATB2; SATB2; Animals; Humans; Developmental; developmental timing; 3' Untranslated Regions; neural stem cells; Cerebral Cortex; Settore BIO/11 - BIOLOGIA MOLECOLARE; cell fate; 0303 health sciences; microRNA; Eutheria; cortical layering; Tumor Suppressor Proteins; miR-catch; Gene Expression Regulation, Developmental; Cell Differentiation; vitro corticogenesis; Matrix Attachment Region Binding Proteins; mammalian evolution; Repressor Proteins; MicroRNAs; cortex; Gene Expression Regulation; in vitro corticogenesis; cell identity; Cell fate; cell identity; corpus callosum; cortex; cortical layering; developmental timing; in vitro corticogenesis; mammalian evolution; microRNA; miR-catch; neural stem cells; post-transcriptional control; SATB2; 3' Untranslated Regions; Animals; Cell Differentiation; Cell Line; Cerebral Cortex; Eutheria; Gene Expression Regulation, Developmental; Humans; Matrix Attachment Region Binding Proteins; Mice; MicroRNAs; Neurogenesis; Repressor Proteins; Transcription Factors; Tumor Suppressor Proteins; Transcription Factors

Cerebral cortical development is controlled by key transcription factors that specify the neuronal identities in different layers. The mechanisms controlling their expression distinct cells are only partially known. We investigated and stability of Tbr1, Bcl11b, Fezf2, Satb2, Cux1 mRNAs single developing mouse cells. observe Satb2 mRNA appears much earlier than its protein a set broader expected, suggesting an initial inhibition translation, subsequently released during development. Mechanistically, 3′UTR modulates translation GFP reporters corticogenesis. select miR-541, eutherian-specific miRNA, miR-92a/b as best candidates responsible for SATB2 inhibition, being strongly expressed early reduced late progenitor Their inactivation triggers robust premature both human Our findings indicate RNA interference major mechanism timing cell identities.