Generation of caudal-type serotonin neurons and hindbrain-fate organoids from hPSCs

• DOI: 10.1016/j.stemcr.2021.06.006
• Publication Date: 2021-07-08T16:23:41Z
• Authors: Parvin Valiulahi; Vincencius Vidyawan; Lesly Puspita; Youjin Oh; Virginia Blessy J...; Panida Sittipo; Gilgi Friedlander; Dayana Yahalomi; Jong-Woo Sohn; Yun Kyung Lee; Jeong Kyo Yoon; Jae-won Shim
• Keywords: Hindbrain; Organoid; Fate mapping

Serotonin (5-HT) neurons, the major components of raphe nuclei, arise from ventral hindbrain progenitors. Based on anatomical location and axonal projection, 5-HT neurons are coarsely divided into rostral caudal groups. Here, we propose a novel strategy to generate human pluripotent stem cells (hPSCs), which involves formation ventral-type neural progenitor stimulation development. A caudalizing agent, retinoid acid, was used direct cell fate. Approximately 30%–40% hPSCs successfully developed 5-HT-expressing using our protocol, with majority acquiring rhombomere identity (r5–8). We further modified monolayer differentiation system neuron-enriched hindbrain-like organoids. also suggest downstream applications organoid cultures study neuronal response gut microbiota. Our methodology could become powerful tool for future studies related neurotransmission.