Development of a scalable method to isolate subsets of stem cell-derived pancreatic islet cells

• DOI: 10.1016/j.stemcr.2022.02.001
• Publication Date: 2022-03-03T15:38:20Z
• Authors: Audrey V. Parent; Sudipta Ashe; Gopika G. Nair; Mei-Lan Li; Jessica Chavez; Jennifer S. Liu; Yongping Zhong; Philip R. Streeter; Matthias Hebrok
• Keywords: Resource; Pluripotent Stem Cells; 0301 basic medicine; Clinical Sciences; 610; regenerative medicine; Regenerative Medicine; pancreatic beta cells; Autoimmune Disease; Islets of Langerhans; 03 medical and health sciences; Stem Cell Research - Nonembryonic - Human; Insulin-Secreting Cells; Insulin Secretion; Humans; Insulin; Stem Cell Research - Embryonic - Human; Metabolic and endocrine; Transplantation; Stem Cell Research - Induced Pluripotent Stem Cell - Human; Stem Cell Research - Induced Pluripotent Stem Cell; 5.2 Cellular and gene therapies; diabetes; directed differentiation; Diabetes; Cell Differentiation; Biological Sciences; Stem Cell Research; 3. Good health; Glucose; Biochemistry and cell biology; Stem Cell Research - Nonembryonic - Non-Human; Biochemistry and Cell Biology; cell therapy; Biotechnology

Cell replacement therapy using β cells derived from stem is a promising alternative to conventional diabetes treatment options. Although current differentiation methods produce glucose-responsive cells, they can also yield populations of undesired endocrine progenitors and other proliferating cell types that might interfere with long-term islet function safety transplanted cells. Here, we describe the generation an array monoclonal antibodies against surface markers selectively label cell-derived A high-throughput screen identified candidates, including three clones mark high proportion in differentiated cultures. scalable magnetic sorting method was developed enrich for human pluripotent (hPSC)-derived these antibodies, leading formation islet-like clusters improved glucose-stimulated insulin secretion reduced growth upon transplantation. This strategy should facilitate large-scale production functional disease modeling therapy.