Soft X-ray tomography to map and quantify organelle interactions at the mesoscale

• DOI: 10.1016/j.str.2022.01.006
• Publication Date: 2022-02-10T15:29:00Z
• Authors: Valentina Loconte; Jitin Singla; Angdi Li; Jian-Hua Chen; Axel Ekman; Gerry McDermott; Andrej Sali; Mark Le Gros; Kate L. White; Carolyn A. Larabell
• Keywords: Organelle

Inter-organelle interactions are a vital part of normal cellular function; however, these have proven difficult to quantify due the range scales encountered in cell biology and throughput limitations traditional imaging approaches. Here, we demonstrate that soft X-ray tomography (SXT) can be used rapidly map ultrastructural reorganization inter-organelle intact cells. SXT takes advantage naturally occurring, differential absorption carbon-rich compounds each organelle. Specifically, use spatiotemporal evolution insulin vesicles their co-localization interaction with mitochondria pancreatic β cells during secretion response different stimuli. We changes morphology, biochemical composition, relative position vesicles. These findings highlight importance comprehensive unbiased mapping at mesoscale characterize would detect other existing methodologies.