A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain

• DOI: 10.1016/j.synbio.2023.02.002
• Publication Date: 2023-02-11T16:24:41Z
• Authors: Yuting Shuai; Yi Ju; Yuanhang Li; Dini Ma; Lan Jiang; Jingyu Zhang; Gao-Yi Tan; Xueting Liu; Shenlin Wang; Lixin Zhang; Guang Liu
• Keywords: Nucleic acid detection

Nucleic acid detection plays a key role in diverse diagnosis and disease control. Currently available nucleic techniques are challenged by trade-offs among speed, simplicity, precision cost. Here, we described novel method, designated SENSOR (Sulfur DNA mediated sensing platform), for rapid detection. was developed from phosphorothioate (PT)-DNA sulfur binding domain (SBD) which specifically binds double-stranded PT-modified DNA. utilizes PT-DNA oligo SBD as targeting module, is linked with split luciferase reporter to generate luminescence signal within 10 min. We tested on synthesized COVID-19 pseudovirus, achieving attomolar sensitivity combined an amplification procedure. Single nucleotide polymorphisms (SNP) could also be discriminated. Indicating new promising technique.