A novel amperometric galactose biosensor based on galactose oxidase-poly(N-glycidylpyrrole-co-pyrrole)

• DOI: 10.1016/j.synthmet.2010.12.025
• Publication Date: 2011-01-18T04:13:24Z
• Authors: Mehmet Şenel; İbrahim Bozgeyik; Emre Çevik; M. Fatih Abasıyanık
• Keywords: Amperometry; Galactose oxidase; Glucose oxidase

Abstract A novel amperometric galactose biosensor was constructed by immobilization of galactose oxidase (GAox) onto poly(N-glycidylpyrrole-co-pyrrole) film. GAox enzyme was immobilized onto the electrochemically prepared novel conducting polymer film by direct one-step covalent attachment without using any coupling agents. The biosensor surface was characterized by FT-IR spectroscopy and atomic force microscopy (AFM). Amperometric response was measured as a function of concentration of galactose, at fixed potential of +0.7 V vs. Ag/AgCl in a phosphate buffered saline (pH 7.5). The mediated hydrogen peroxide biosensor had a fast response of less than 5 s with linear range 2–16 mM. The sensitivity of the biosensor for galactose was 1.75 μA/mM. The factors influencing on the performance of the resulting biosensor were studied in detail.