Neutrophil extracellular traps: A novel therapeutic target for intracranial hemorrhage

• DOI: 10.1016/j.thromres.2022.08.024
• Publication Date: 2022-08-28T01:10:14Z
• Authors: Jiaqi Jin; Xinyi Zhao; Wenqiang Li; Fang Wang; Jiawei Tian; Nan Wang; Xin Gao; Jiheng Zhang; Jiasheng Wu; Ge Mang; Ruishuang Ma; Shaoshan Hu
• Keywords: Histones; 0301 basic medicine; Hematoma; 03 medical and health sciences; Neutrophils; Deoxyribonuclease I; Humans; DNA; Extracellular Traps; Biomarkers; Cerebral Hemorrhage; 3. Good health

Neutrophil extracellular traps (NETs) are known to play a role in various diseases affecting coagulation. As of now, it is unclear whether NETs are present in hematoma samples collected from patients who have suffered an intracranial hemorrhage (ICH). The objective of this was to determine whether NETs are present in circulation and hematoma samples from ICH patients and to evaluate the procoagulant activity (PCA) of NETs during the ICH process. The expression of NET markers in samples from 78 ICH patients and 35 healthy donners was detected by ELISA and flow cytometry. Immunostaining for neutrophil markers (neutrophil CD66b) and NET markers (citrullinated histone H3 [H3Cit] and extracellular DNA) was performed on hematoma samples obtained from ICH patients undergoing intracranial hematoma evacuation. Our findings suggest that plasma and hematoma samples from patients with ICH showed high levels of NETs. Furthermore, using DNase I to target NETs enhanced ex vivo hematoma lysis. In conclusion, NETs play an important role in the ICH process and may be a novel therapeutic target for treatment of ICH patients.