Biological response and morphological assessment of individually dispersed multi-wall carbon nanotubes in the lung after intratracheal instillation in rats

• DOI: 10.1016/j.tox.2010.07.021
• Publication Date: 2010-08-08T08:46:22Z
• Authors: Norihiro Kobayashi; Masato Naya; Makoto Ema; Shigehisa Endoh; Junko Maru; Kohei Mizuno; Junko Nakanishi
• Keywords: Alveolar Wall; Intratracheal instillation

Biological responses of multi-wall carbon nanotubes (MWCNTs) were assessed after a single intratracheal instillation in rats. The diameter and median length the MWCNTs used this study approximately 60 nm 1.5 μm, respectively. Groups male Sprague–Dawley rats intratracheally instilled with 0.04, 0.2, or 1 mg/kg individually dispersed MWCNT suspension. After instillation, bronchoalveolar lavage fluid was for inflammatory cells markers, lung, liver, kidney, spleen, cerebrum histopathologically evaluated at 3-day, 1-week, 1-month, 3-month, 6-month post-exposure. Transient pulmonary observed only lungs group exposed to MWCNTs. Morphology using light microscopy transmission electron (TEM). Light examination revealed that deposited typically phagocytosed by alveolar macrophages these consequently accumulated alveoli until 400 TEM images obtained showed all located interstitial tissues, not tissues. There no evidence chronic inflammation, such as angiogenesis fibrosis, induced instillation. These results suggest being processed cleared macrophages.