Adipose mesenchymal stem cell-derived antioxidative extracellular vesicles exhibit anti-oxidative stress and immunomodulatory effects under PM2.5 exposure

• DOI: 10.1016/j.tox.2020.152627
• Publication Date: 2020-11-05T15:21:36Z
• Authors: Yongheng Gao; Xia Huang; Hongwei Lin; Mingxuan Zhao; Wei Liu; Wangping Li; Luyao Han; Qiong Ma; Chuan Dong; Yujuan Li; Ying Hu; Faguang Jin
• Keywords: 0301 basic medicine; Acute Lung Injury; Mesenchymal Stem Cell Transplantation; Antioxidants; Rats; 3. Good health; Immunomodulation; Rats, Sprague-Dawley; Extracellular Vesicles; Oxidative Stress; Random Allocation; 03 medical and health sciences; Adipose Tissue; Animals; Particulate Matter; Reactive Oxygen Species

PM2.5 exposure elevates the level of reactive oxygen species (ROS) in lungs and leads to lung injury or other pulmonary conditions. Nrf2 is a key antioxidative regulator that suppresses ROS production. Extracellular vesicles (EVs) secreted by adipose mesenchymal stem cells (ADSCs) have been identified as therapeutic well potential drug/gene/protein carriers. In this study, we established rat (PM2.5, 100 μL, 5 mg/mL) cell 50 μg/mL) models conduct vivo vitro studies on adverse effects PM2.5. Our findings indicated initial responses were robust oxidative stress inflammation. EVs (Antioxi-EVs, derived from ADSCs overexpress Nrf2) had tested interventions PM2.5-treated through tracheal instillation co-incubation. Treatment with Antioxi-EVs (3 × 1010 particles 1 109 vitro) was found suppressive effect levels inflammatory cytokines, having superior anti-oxidative stress. particular, occurrence apoptosis correlated positively level, inhibition upregulating alleviated apoptosis. Furthermore, treatment increased M2-like macrophages compared PBS further reduced IL-6 TNF-α levels. results suggest can reduce severity stress, inflammation, induced via immunomodulation pathways.