Proteomics and histological assessment of an organotypic model of human skin following exposure to Naja nigricollis venom

• DOI: 10.1016/j.toxicon.2022.106955
• Publication Date: 2022-10-27T01:15:43Z
• Authors: Shirin Ahmadi; Spyridon T. Pachis; Konstantinos Kalo...; Farrell McGeoghan; Vahap Canbay; Steven R. Hall; Edouard P. Critte...; Charlotte A. Dawson; Keirah E. Bartlett; José María Gutiérrez; Nicholas R. Casewell; Ulrich auf dem Ke...; Andreas H. Laustsen
• Keywords: Naja; Animal testing; Ex vivo; Animal model

Snakebite envenoming was reintroduced as a Category A Neglected Tropical Disease by the World Health Organization in 2017. Since then, increased attention has been directed towards this affliction and development of deeper understanding how snake venoms exert their toxic effects antivenoms can counter them. However, most our vivo generated knowledge stems from use animal models which do not always accurately reflect pathogenic manifest humans. Moreover, experiments are associated with pain, distress, eventually sacrifice due to nature venoms. Related this, implementation 3Rs principle (Replacement, Reduction, Refinement) experimental animals snakebite research is recommended Organization. Therefore, more humane designs new vitro/ex alternatives for sought after. Here, we report an organotypic model human skin further elucidate pathophysiology dermonecrotic caused venom black-necked spitting cobra, Naja nigricollis, The goal study expand repertoire available that be used local tissue damages induced cytotoxic