New insights into the human metabolism of the Fusarium mycotoxins deoxynivalenol and zearalenone

• DOI: 10.1016/j.toxlet.2013.04.012
• Publication Date: 2013-04-23T19:05:26Z
• Authors: Benedikt Warth; Michael Sulyok; Franz Berthiller; Rainer Schuhmacher; Rudolf Krska
• Keywords: Glucuronide

This study reports on the detailed investigation of human deoxynivalenol (DON) and zearalenone (ZEN) in vivo metabolism through analysis urine samples obtained from one volunteer following a naturally contaminated diet containing 138μg DON 10μg ZEN over period four days. Based mycotoxin intake concentrations conjugates urine, mass balance was established. The average rates excretion glucuronidation were determined to be 68 76%, respectively. formed glucuronides revealed DON-15-glucuronide as main conjugation product besides DON-3-glucuronide. Furthermore, for first time third DON-glucuronide detected fate ingested masked forms (3-acetyl-DON DON-3-glucoside) preliminary assessed. mean rate 9.4%. mainly present its glucuronide form some ZEN-14-glucuronide directly 3-10h after exposure. For concrete figures have become available pattern ZEN-glucuronides throughout day, comparison total 24h morning urinary humans exposure food. Therefore, valuable information has been chosen experimental approach although involved only single individual needs confirmed larger monitoring studies. presented experiment contributes better understanding thereby supports advanced risk assessment increase food safety examine relationship between these mycotoxins potentially associated chronic diseases future.