Serum proteomic analysis reveals potential serum biomarkers for occupational medicamentosa-like dermatitis caused by trichloroethylene

• DOI: 10.1016/j.toxlet.2014.05.024
• Publication Date: 2014-06-21T16:48:08Z
• Authors: Peiwu Huang; Xiaohu Ren; Zhijun Huang; Xifei Yang; Wenxu Hong; Yanfang Zhang; Hang Zhang; Wei Liu; Haiyan Huang; Xinfeng Huang; Desheng Wu; Linqing Yang; Haiyan Tang; Li Zhou; Xuan Li; Jianjun Liu
• Keywords: Adult; Male; Proteomics; 0301 basic medicine; Adolescent; Blotting, Western; Gene Expression; Enzyme-Linked Immunosorbent Assay; Blood Proteins; Trichloroethylene; 3. Good health; Occupational Diseases; Young Adult; 03 medical and health sciences; Liver Function Tests; Echocardiography; Occupational Exposure; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Databases, Genetic; Humans; Female; Drug Eruptions; Biomarkers

Trichloroethylene (TCE) is an industrial solvent with widespread occupational exposure and also a major environmental contaminant. Occupational medicamentosa-like dermatitis induced by trichloroethylene (OMLDT) is an autoimmune disease and it has become one major hazard in China. In this study, sera from 3 healthy controls and 3 OMLDT patients at different disease stages were used for a screening study by 2D-DIGE and MALDI-TOF-MS/MS. Eight proteins including transthyretin (TTR), retinol binding protein 4 (RBP4), haptoglobin, clusterin, serum amyloid A protein (SAA), apolipoprotein A-I, apolipoprotein C-III and apolipoprotein C-II were found to be significantly altered among the healthy, acute-stage, healing-stage and healed-stage groups. Specifically, the altered expression of TTR, RBP4 and haptoglobin were further validated by Western blot analysis and ELISA. Our data not only suggested that TTR, RBP4 and haptoglobin could serve as potential serum biomarkers of OMLDT, but also indicated that measurement of TTR, RBP4 and haptoglobin or their combination could help aid in the diagnosis, monitoring the progression and therapy of the disease.