UVB damage onset and progression 24 h post exposure in human-derived skin cells

• DOI: 10.1016/j.toxrep.2017.07.008
• Publication Date: 2017-08-01T16:17:57Z
• Authors: Christian Khalil; Wassim Shebaby
• Keywords: Comet Assay; HaCaT; Cell damage

The focus of this research was on UVB radiation (280-320 nm) responsible for cellular changes in skin acute and chronically exposed individuals. This study investigated the damages triggered by exposure cultured human fibroblasts keratinocyte cells immediately 24 h post order to understand damage onset progression. evaluated a number parameters including mitochondria, lysosomes, cell membrane, DNA as well pro anti-apoptotic protein expression levels. Cellular organelle were assessed battery vitro toxicological assays using MTS Neutral red cytotoxicity assays. Cell membrane also measuring lactate dehydrogenase (LDH) enzyme leakage from cells. Lastly comet assay while Western Blot. In we reported all our systems (MTS, NR LDH) that dose dependent with amplified exposure. Our results indicated incubation period increased sensitivity used. detecting early cytotoxic manifested though measurement at very low doses which not data HaCaT most sensitive assay. We established upregulation downregulation various apoptotic proteins time points presented clearly need comprehensive assessment 4 due different sensitivities addition signaling mechanisms activated