Bisphenols, but not phthalate esters, modulate gene expression in activated human MAIT cells in vitro

• DOI: 10.1016/j.toxrep.2023.02.017
• Publication Date: 2023-03-01T23:08:21Z
• Authors: J.L. Krause; A. Pierzchalski; H.D. Chang; A.C. Zenclussen; M. Bauer; G. Herberth
• Keywords: Phthalate ester; Immunomodulation; In vitro model; Plasticizers; RA1190-1270; Toxicology. Poisons; MAIT cells; Bisphenols; Article

One route of human exposure to environmental chemicals is oral uptake. This primarily true for that may leach from food packaging materials, such as bisphenols and phthalate esters. Upon ingestion, these compounds are transported along the intestinal tract, where they can be taken up into blood stream or distributed mucosal sites. At sites, immune cells in peripheral exposed potentially modulating cell functions. In present study, we investigated impact three common two esters on mucosal-associated invariant T (MAIT) vitro, a frequent type mucosae humans. All were non-cytotoxic at chosen concentrations. MAIT activation was only slightly affected seen by flow cytometric analysis. Phthalate did not affect gene expression, while bisphenol-exposure induced significant changes. Transcriptional changes occurred ∼ 25 % genes BPA, 22 BPF 8 BPS. down-modulated expression CCND2, CCL20, GZMB IRF4, indicating an effect effector function. Further, BPA showed high overlap modulated involved cellular stress response, signaling function suggesting safe substitute BPA.