RNA-binding protein IGF2BP2 suppresses metastasis of clear cell renal cell carcinoma by enhancing CKB mRNA stability and expression

• DOI: 10.1016/j.tranon.2024.101904
• Publication Date: 2024-02-10T04:42:58Z
• Authors: Junwu Ren; Bo Huang; Wei Li; Yongquan Wang; Xiaojuan Pan; Qiang Ma; Yuying Liu; Xiaolin Wang; Ce Liang; Yuying Zhang; Shimin Wang; Feifei Yang; Haiping Li; Hao Ning; Yan Jiang; Changhong Qin; Ai Ran; Bin Xiao
• Keywords: Clear cell renal cell carcinoma; 0301 basic medicine; 0303 health sciences; IGF2BP2; CKB; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; mRNA stability; RNA binding protein; RC254-282; Original Research

Clear cell renal carcinoma (ccRCC) is the most prevalent kidney cancer, with a highly aggressive phenotype and poor prognosis. RNA binding proteins (RBPs) play crucial roles in post-transcriptional gene regulation have been implicated tumorigenesis. RBPs potential to become new therapeutic target for ccRCC. In this study, we screened validated that insulin-like growth factor 2 mRNA protein (IGF2BP2) as an RBP, was down-regulated ccRCC tissues lines. Functionally, verified IGF2BP2 significantly suppressed migration invasion ability of vitro vivo. Mechanistically, RIP-seq actinomycin D experiments results showed enhanced expression Creatine Kinase B (CKB) by CKB enhancing its stability. Thus, inhibited metastasis through CKB. Taken together, these finding suggests novel suppressor may serve target.