Knockdown of YTHDF2 initiates ERS-induced apoptosis and cancer stemness suppression by sustaining GLI2 stability in cervical cancer

• DOI: 10.1016/j.tranon.2024.101994
• Publication Date: 2024-05-21T22:40:51Z
• Authors: Fujian Wan; Fengwu Qiu; Yang Deng; Hao Hu; Yingjie Zhang; Jia-Yu Zhang; Pei Kuang; Haoyu Tian; Dewang Wu; Hang Min; Jiapeng Li; Jing Xu; Jun Zhou
• Keywords: 0301 basic medicine; 0303 health sciences; YTHDF2; Cervical cancer; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; Apoptosis; ERS; Tumor stemness; JNK; RC254-282; Original Research

Cervical cancer ranks fourth in women terms of incidence and mortality. The RNA-binding protein YTH N6-methyladenosine F2 (YTHDF2) contributes to progression by incompletely understood mechanisms. We show how YTHDF2 controls the fate cervical cells whether could be a valid target for therapy cancer. Sphere formation alkaline phosphatase staining assays were performed evaluate tumor stemness following knockdown. Apoptosis was detected flow cytometry TUNEL assay. compounds 4PBA SP600125 used investigate correlation between JNK, endoplasmic reticulum stress, stemness, apoptosis. Data from Cancer Genome Atlas (TCGA) databases Gene Expression Omnibus (GEO) revealed that GLI family zinc finger 2 (GLI2) might YTHDF2. transcription inhibitor actinomycin D dual-luciferase reporter gene employed association GLI2 mRNA Nude mouse xenografts generated assess effects knockdown on growth vivo. Knockdown up-regulated expression GLI2, leading JNK phosphorylation stress. These processes inhibited proliferation their cell promotion In conclusion, significantly affects cells, making it potential treating