Using the Patterned Microarray Culture to Obtain Gene-Editing Monoclonal Cells

• DOI: 10.1016/j.transproceed.2020.02.138
• Publication Date: 2020-05-24T11:15:01Z
• Authors: Yunlin Zhang; Mengyu Gao; Meijuan Hu; Bingqi Zhang; Yuting He; Shun Li; Ji Bao; Hong Bu
• Keywords: Gene Editing; 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Swine; Cell Culture Techniques; Animals; Microarray Analysis; Clone Cells

Genetically modified pigs are the first choice for xenotransplantation research, but there have been problems with monoclonal screening of edited cells before nuclear transfer. Our objective was to get a novel strategy to quickly obtain monoclonal cells with low damage by microarray and to produce efficient gene-editing monoclonal cells in batches. Micropattern array printing technology was introduced to limit only a single cell was adhered on a micropattern substrate, and after 4 days of culture, the single cell grew into a monoclonal cell sphere and then came off from the bottom of the petri dish automatically. After sequencing, the results showed that a single cell is confined to a micropattern and grows into a sphere of monoclonal cells.