Using the Patterned Microarray Culture to Obtain Gene-Editing Monoclonal Cells

• DOI: 10.1016/j.transproceed.2020.02.138
• Publication Date: 2020-05-24T11:15:01Z
• Authors: Yunlin Zhang; Mengyu Gao; Meijuan Hu; Bingqi Zhang; Yuting He; Shun Li; Ji Bao; Hong Bu
• Keywords: Petri dish; Xenotransplantation

Genetically modified pigs are the first choice for xenotransplantation research, but there have been problems with monoclonal screening of edited cells before nuclear transfer. Our objective was to get a novel strategy to quickly obtain monoclonal cells with low damage by microarray and to produce efficient gene-editing monoclonal cells in batches. Micropattern array printing technology was introduced to limit only a single cell was adhered on a micropattern substrate, and after 4 days of culture, the single cell grew into a monoclonal cell sphere and then came off from the bottom of the petri dish automatically. After sequencing, the results showed that a single cell is confined to a micropattern and grows into a sphere of monoclonal cells.