TIPE3 protects mice from lipopolysaccharide-induced acute lung injury

• DOI: 10.1016/j.trim.2023.101799
• Publication Date: 2023-02-24T14:33:00Z
• Authors: Jie Song; Qiuping Yang; Hui Xiong; Xia Gu; Mo Chen; Chuanxin Zhou; Yao Cai
• Keywords: Lipopolysaccharides; Inflammation; 0301 basic medicine; Acute Lung Injury; NF-kappa B; Intracellular Signaling Peptides and Proteins; 3. Good health; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Animals; Carrier Proteins; Lung

Acute lung injury (ALI) is a severe inflammatory disease with high morbidity and mortality in patients and lung transplant recipients. Tumor necrosis factor-α-induced protein 8-like 3 (TIPE3) is one of the members of the TIPE family. While TIPE2 has been demonstrated to be protective against lipopolysaccharide (LPS)-induced ALI, the role of TIPE3 in ALI is currently unidentified.To examine the role of TIPE3 in ALI, we pretreated C57BL/6 mice with control or TIPE3-lentivirus in LPS-induced ALI models. The C57BL/6 mice were randomly divided into four groups: control group; ALI-induced group; ALI-induced group with control lentivirus; and ALI-induced group with TIPE3-lentivirus. Additionally, RAW 264.7 cells were used to validate the role and molecular mechanism of TIPE3 signaling in vitro.An increased expression of TIPE3 reduced lung histopathological damage in ALI-affected mice. ALI-affected mice treated with TIPE3-lentivirus exhibited reduced lung microvascular permeability, myeloperoxidase (MPO) activity, neutrophil buildup, and inflammation response. Additionally, over-expression of TIPE3 significantly inhibited NF-κB activation and promoted the activation of Liver X receptors alpha (LXRα). In LPS-treated RAW264.7 cells, enforced TIPE3 expression produced anti-inflammatory effects, whereas the LXR inhibitor geranylgeranyl pyrophosphate (GGPP) reversed these effects.TIPE3 protected against LPS-induced ALI by regulating the LXRα/NF-κB signaling pathway. These results suggest that TIPE3 might provide a novel insight into the prevention of ALI.