Evaluation of neuroendocrine staining and androgen receptor expression in incidental prostatic adenocarcinoma: Prognostic implications

• DOI: 10.1016/j.urology.2005.04.064
• Publication Date: 2005-10-18T11:20:50Z
• Authors: V.E. Theodoropoulos; A. Tsigka; A. Mihalopoulou; V. Tsoukala; A.C. Lazaris; E. Patsouris; I. Ghikonti
• Keywords: Prostatic adenocarcinoma; Neuroendocrine differentiation

To identify neuroendocrine cells and androgen receptors (ARs), possible predictors of cancer progression, in a series of untreated patients with incidental Stage T1a prostate cancer (PCa). Neuroendocrine cells may exert a dynamic role in the microenvironment of PCa. The AR is thought to have a central role in the propagation of prostate carcinogenesis.Prostatectomy specimens from 81 patients with Stage T1a PCa were available for analysis. Neuroendocrine cells were detected by immunohistochemistry using antibodies to chromogranin A (CgA) and neuron-specific enolase, and the antibody against AR enabled the evaluation of the nuclear AR status. Tumor cell proliferation was assessed with the Ki-67 labeling index using MIB-1 antibody. The patients were followed up for a mean of 63.9 months, and a subsequent rise in prostate-specific antigen or positive digital rectal examination findings confirmed by biopsy was considered disease progression.Of the 81 specimens, 62 (76.54%) were positive for CgA and/or neuron-specific enolase and 19 (23.46%) were negative. A statistically significant correlation was found between CgA positivity and tumor dedifferentiation (P = 0.002). Well-differentiated tumors revealed an overexpression of ARs (P < 0.005). On multivariate analysis, worsening tumor differentiation emerged as the only independent predictor of progression-free survival (P = 0.041); however, only CgA positivity was an independent predictor of tumor progression in well and moderately differentiated tumors (P = 0.038).The results of this study suggest that CgA may represent a useful marker for subsequent aggressive behavior and progression in incidental well and moderately differentiated Stage T1a PCa.