Analysis of the immunogenicity and bioactivities of a split influenza A/H7N9 vaccine mixed with MF59 adjuvant in BALB/c mice

• DOI: 10.1016/j.vaccine.2016.03.037
• Publication Date: 2016-03-22T07:23:48Z
• Authors: Huilin Ou; Hangping Yao; Wei Yao; Nanping Wu; Xiaoxin Wu; Chengcong Han; Linfang Cheng; Keda Chen; Honglin Chen; Lanjuan Li
• Keywords: Antibody titer

The H7N9 influenza virus caused significant mortality and morbidity in humans during an outbreak China 2013. A recombinant seed with hemagglutinin (HA) neuraminidase (NA) gene segments from A/Zhejiang/DTID-ZJU01/2013(H7N9) six internal protein A/Puerto Rico/8/34(H1N1; PR8) were generated using reverse genetics. We sought to determine the immunogenic, protective properties, mechanisms of a split avian A/H7N9 vaccine mixed MF59 adjuvant comparison vaccines that included other adjuvant. BALB/c mice vaccinated two doses different amounts combinations this novel A/ZJU01/PR8/2013 Mice subsequently challenged by intranasal inoculation. verified enhanced HI, MN, IgG antibody titers antigens. Compared alum, could more potentially induce humoral immune responses Th2 cytokine production after infection, while both alum can slightly increase NK cell activity. This effectively protect challenge. have selected for manufacture future clinical studies infection.