Sucrose-based cryoprotective storage of extracellular vesicles

• DOI: 10.1016/j.vesic.2022.100016
• Publication Date: 2022-11-21T11:59:04Z
• Authors: Sierra A. Walker; Irina Davidovich; Yubo Yang; Andrew Lai; Jenifer Pendiuk G...; Vatsal Deliwala; Sara Busatto; Shane Shapiro; Na’ama Koifman; Carlos Salomon; Yeshayahu Talmon; Joy Wolfram
• Keywords: Cryoprotectant; Phosphate buffered saline; Extracellular Vesicles

Advancements in extracellular vesicle (EV) studies necessitate the development of optimized storage conditions to ensure preservation physical and biochemical characteristics. In this study, most common buffer for EV (phosphate-buffered saline/PBS) was compared a cryoprotective 5% sucrose solution. The size distribution concentration EVs from two different sources changed greater extent after -80 °C PBS Additionally, molecular surface protrusions transmembrane proteins were more prevalent stored solution those PBS. This study demonstrates, first time, that distinct ring-like complexes cristae-like folded membranous structures are visible upon degradation. Taken together, size, concentration, extensions, varied substantially based on used storage, suggesting biocompatible cryoprotectants, such as sucrose, should be considered studies.