Analysis of Influenza A virus infection in human induced pluripotent stem cells (hiPSCs) and their derivatives

• DOI: 10.1016/j.virusres.2022.199009
• Publication Date: 2022-11-19T23:31:59Z
• Authors: Kanyarat Ruangrung; Warunya Chakritbu...; Songkran Thongon; Sasitorn Rungarun...; Methichit Wattana...; Chompunuch Boonar...; Ornpreya Suptawiwat; Naraporn Sirinont...; Duncan Richard Smith; Prasert Auewarakul
• Keywords: Permissiveness; Germ layer; Sendai virus; Viral infection

Influenza A virus (IAV) infection in pregnant women is a major public health concern. However, the effect of IAV on human embryogenesis still unclear. Here we show that induced pluripotent stem cells (hiPSCs) and hiPSC-derived ectodermal, mesodermal endodermal are susceptible to infection. These cell types stained positive for α2,6-linked sialic acid, receptor expressed surface. While hiPSCs produced high viral titers up 7 days with increasing infected number suggesting progenies from without exogenous protease were infectious could spread other cells, three germ-layer showed decline lack spreading. Amongst germ layers, less than ectodermal cells. results indicate permissiveness early embryogenesis, suggest risk detrimental effects embryonic development.