Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic individuals with COVID-19

• DOI: 10.1016/j.xcrm.2021.100193
• Publication Date: 2021-01-18T08:01:57Z
• Authors: Yun Shan Goh; Jean-Marc Chavatte; Alicia Lim Jieling; Bernett Lee; Pei Xiang Hor; Siti Naqiah Amrun; Cheryl Yi-Pin Lee; Rhonda Sin-Ling Chee; Bei Wang; Chia Yin Lee; Eve Zhi Xian Ngoh; Cheng-I Wang; Barnaby Edward Young; Paul A. Tambyah; Shirin Kalimuddin; Surinder Pada; Seow-Yen Tan; Louisa Jin Sun; Mark I-Cheng Chen; Yee-Sin Leo; David C. Lye; Lisa F.P. Ng; Raymond Tzer Pin Lin; Laurent Renia
• Keywords: Isotype; Asymptomatic carrier; Coronavirus; Immunoglobulin M

Early detection of infection is crucial to limit the spread coronavirus disease 2019 (COVID-19). Here we develop a flow cytometry-based assay detect severe acute respiratory syndrome 2 (SARS-CoV-2) spike (S) protein antibodies in individuals with COVID-19. The detects specific immunoglobulin M (IgM), IgA, and IgG COVID-19 also acquisition all subclasses, IgG1 being most dominant. antibody response significantly higher at later stage infection. Furthermore, asymptomatic IgM, IgG, dominant subclass. Although levels are lower infection, highly sensitive 97% infections. These findings demonstrate that can be used for serological analysis symptomatic infections, which may otherwise remain undetected.