Single-molecule nanopore dielectrophoretic trapping of α-synuclein with lipid membranes

• DOI: 10.1016/j.xcrp.2022.101243
• Publication Date: 2023-01-18T17:27:23Z
• Authors: Jinming Wu; Tohru Yamashita; Andrew D. Hamilton; Sam Thompson; Jinghui Luo
• Keywords: Nanopore; Conformational change

The lipid-α-Synuclein (α-Syn) interaction plays a crucial role in the pathogenesis of Parkinson's disease. Here, we investigate lipid-binding and -unbinding kinetics α-Syn an α-hemolysin (αHL) single nanopore. Under applied voltage, engineered sequence can be trapped at nanopore due to dielectrophoretic force. conformational switch events observed pore-membrane junction through interpretation blockade current amplitudes dwell time. This allows further analysis dynamics. We study how disease-associated metal ions (Cu2+, Zn2+) modulate dynamics interface membranes pore α-helical peptidomimetics stabilize helical conformation lipidic environment. These studies aid our understanding complexity α-Syn, lipid membranes, ions, using peptidomimetics, new strategy against toxicity aggregation is advanced.