Whole-genome resequencing identifies candidate genes and allelic variation in the MdNADP-ME promoter that regulate fruit malate and fructose contents in apple

• DOI: 10.1016/j.xplc.2024.100973
• Publication Date: 2024-05-14T16:37:14Z
• Authors: Weihong Fu; Lin Zhao; Wanjun Qiu; Xu Xu; Meng Ding; Liming Lan; Shenchun Qu; Sanhong Wang
• Keywords: Candidate gene; Genetic linkage

Soluble sugar and organic acids are key determinants of fruit organoleptic quality directly affect the commodity value economic returns crops. We performed whole-genome sequencing apple varieties Gala Xiahongrou, along with their F1 hybrids, to construct a high-density bin map. Our quantitative genetic analysis pinpointed 53 trait loci (QTLs) related 11 acid traits. identified candidate gene, MdNADP-ME, responsible for malate degradation, in stable QTL on linkage group 15. Sequence revealed an A/C SNP promoter region (MEp-799) that influences binding MdMYB2 transcription factor, thereby affecting MdNADP-ME expression. In our study various genotypes, this has been demonstrated be linked fructose levels. also developed dCAPS marker associated content. These results substantiate role maintaining equilibrium between contents fruits.