Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractions

• DOI: 10.1016/j.xpro.2021.100628
• Publication Date: 2021-06-23T23:59:03Z
• Authors: Mamta Rai; Michelle Curley; Zane Coleman; Anjana Nityanandam; Jianqin Jiao; Flavia A. Graca; Liam C. Hunt; Fabio Demontis
• Keywords: Proteostasis

Defects in protein quality control are the underlying cause of age-related diseases. The western blot analysis detergent-soluble and insoluble fractions has proven useful identifying interventions that regulate proteostasis. Here, we describe protocol for such analyses Drosophila tissues, mouse skeletal muscle, human organoids, HEK293 cells. We key adaptations this provide information will help modify future studies other tissues disease models. For complete details on use execution protocol, please refer to Rai et al. (2021) Hunt el (2021).