Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings

• DOI: 10.1016/j.xpro.2021.100922
• Publication Date: 2021-10-29T03:18:30Z
• Authors: Yanlin Liu; Yan Xiong
• Keywords: 2. Zero hunger; 0301 basic medicine; Science (General); Nitrogen; TOR Serine-Threonine Kinases; Arabidopsis; Cell Biology; Carbon; Culture Media; Tissue Culture Techniques; Q1-390; 03 medical and health sciences; Model Organisms; Seedlings; Developmental biology; Protocol; Plant sciences; Signal Transduction

Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficient depletion of the endogenous carbon/nitrogen nutrients in Arabidopsis seedlings. Exogenous carbon/nitrogen can be supplied to dissect the TOR pathway. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2013) and Liu et al. (2021).