Medium throughput protocol for genome-based quantification of intracellular mycobacterial loads and macrophage survival during in vitro infection

• DOI: 10.1016/j.xpro.2022.101241
• Publication Date: 2022-03-15T23:52:04Z
• Authors: Shivraj M. Yabaji; Sujoy Chatterjee; Emily Waligursky; Alexander Gimelbrant; Igor Kramnik
• Keywords: Viability assay

Here, we present a streamlined protocol for assessing intracellular Mycobacterium tuberculosis (Mtb) loads in macrophages. This describes the simultaneous assessment of macrophage viability using automated microscopy. Further, detail quantification mycobacterial rapid, inexpensive, and accurate approach DNA isolation from paraformaldehyde-fixed Simultaneous bacterial internal standard allows precise effects perturbations on Mtb host cells while accounting technical artifacts. For complete details use execution this protocol, please refer to Chatterjee et al. (2021).