Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models

• DOI: 10.1016/j.xpro.2022.101855
• Publication Date: 2022-11-16T23:03:24Z
• Authors: Bárbara Jonson Ba...; Larisse Longo; Valessa Emanoele ...; Fernando Salvati; Carolina Uribe-Cruz; Guido Lenz; Ricardo Machado X...; Mário Reis Álvare...; Fernanda Visioli; Eduardo Cremonese...
• Keywords: 0301 basic medicine; Microscopy; Q1-390; Mice; Disease Models, Animal; 03 medical and health sciences; Science (General); Tissue Engineering; Developmental biology; Muscle Fibers, Skeletal; Protocol; Animals

The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers' subpopulations, aiming to characterize physiopathological conditions related to muscle tissue. The main limitation of MusMA is the need for high-quality tissue slides and images and control samples to set up the analyses.