Protocol for evaluating mitochondrial respiration in iPSC-derived neurons by the Seahorse XF analyzer

• DOI: 10.1016/j.xpro.2024.103127
• Publication Date: 2024-07-15T10:43:00Z
• Authors: Chiara Fasano; Andrea Cavaliere; Valeria Tiranti; Camille Peron
• Keywords: Respiration rate; Seahorse; Normalization

Here, we present a protocol describing the quantification of oxygen consumption rate (OCR) and maximal respiration (MRR) in living induced pluripotent stem cell (iPSC)-derived neurons using Seahorse analyzer. We guide you through whole process: culture amplification seeding neural progenitor cells (NPCs), their differentiation into neurons, normalization results to number analytical phase. The assessment cellular mitochondrial function, by analyzing respiration, could be useful various diseases as well drug screening. For complete details on use execution this protocol, please refer Aleo et al.