Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus

• DOI: 10.1016/j.xpro.2025.103627
• Publication Date: 2025-02-27T18:47:57Z
• Authors: Lillian I.J. Byer; Huixin Xu; Maria K. Lehtinen
• Keywords: immunology; neuroscience; Q1-390; Science (General); cell biology; microscopy; Protocol

Extracting the choroid plexus (ChP) for histology provides a comprehensive view of the whole tissue and is crucial for understanding its structure and cellular interactions. Here, we present a protocol for dissecting out the lateral, third, and fourth ventricle ChP from mice and the immunostaining process. We also detail procedures for preparing whole-mount ChP tissue and imaging techniques, such as tile scan and Z-stacks. For complete details on the use and execution of this protocol, please refer to Xu et al.(1)