Identification of C. elegans sensory ray genes using whole-genome expression profiling
Male
Polycystins
Sensory Receptor Cells
Proneural
E-Box Elements
03 medical and health sciences
Genes, Reporter
Databases, Genetic
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Molecular Biology
Oligonucleotide Array Sequence Analysis
0303 health sciences
Neural subtype
Gene Expression Profiling
Cell Differentiation
Cell Biology
Genetic network
Male tail
C. elegans
lin-32
DNA microarrays
Sensory rays
Developmental Biology
Transcription Factors
DOI:
10.1016/j.ydbio.2004.02.020
Publication Date:
2004-04-03T10:19:28Z
AUTHORS (2)
ABSTRACT
The three cells that comprise each C. elegans sensory ray (two sensory neurons and a structural cell) descend from a single neuroblast precursor cell. The atonal ortholog lin-32 and the E/daughterless ortholog hlh-2 act to confer neural competence during ray development, but additional regulatory factors that control specific aspects of cell fate are largely unknown. Here, we use full-genome DNA microarrays to compare gene expression profiles in adult males of two mutant strains to identify new components of the regulatory network that controls ray development and function. This approach identified a large set of candidate ray genes. Using reporter genes, we confirmed ray expression for 13 of these, including a beta-tubulin, a TWK-family channel, a putative chemoreceptor and four novel genes (the cwp genes) with a potential role in sensory signaling through the C. elegans polycystins lov-1 and pkd-2. Additionally, we have found several ray-expressed transcription factors, including the Zn-finger factor egl-46 and the bHLH gene hlh-10. The expression of many of these genes requires lin-32 function, though this requirement may not reflect direct activation by lin-32. Our strategy provides a complementary foundation for modeling the genetic network that controls the development of a simple sensory organ.
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