Differential gene expression and functional analysis implicate novel mechanisms in enteric nervous system precursor migration and neuritogenesis
0301 basic medicine
Cadherin Related Proteins
Gene Expression
Enteric Nervous System
Epithelium
Mice
03 medical and health sciences
Organ Culture Techniques
Bird
Cell Movement
Morphogenesis
Neurites
Animals
Hirschsprung Disease
Intestinal Mucosa
Protein Precursors
Molecular Biology
Cells, Cultured
Oligonucleotide Array Sequence Analysis
Mice, Knockout
0303 health sciences
Enteric nervous system development
Proto-Oncogene Proteins c-ret
Cell Biology
Cadherins
3. Good health
Mice, Inbred C57BL
Chromosome Pairing
SNARE
Neural crest migration
Developmental Biology
DOI:
10.1016/j.ydbio.2006.06.033
Publication Date:
2006-07-12T13:08:37Z
AUTHORS (9)
ABSTRACT
Enteric nervous system (ENS) development requires complex interactions between migrating neural-crest-derived cells and the intestinal microenvironment. Although some molecules influencing ENS development are known, many aspects remain poorly understood. To identify additional molecules critical for ENS development, we used DNA microarray, quantitative real-time PCR and in situ hybridization to compare gene expression in E14 and P0 aganglionic or wild type mouse intestine. Eighty-three genes were identified with at least two-fold higher expression in wild type than aganglionic bowel. ENS expression was verified for 39 of 42 selected genes by in situ hybridization. Additionally, nine identified genes had higher levels in aganglionic bowel than in WT animals suggesting that intestinal innervation may influence gene expression in adjacent cells. Strikingly, many synaptic function genes were expressed at E14, a time when the ENS is not needed for survival. To test for developmental roles for these genes, we used pharmacologic inhibitors of Snap25 or vesicle-associated membrane protein (VAMP)/synaptobrevin and found reduced neural-crest-derived cell migration and decreased neurite extension from ENS precursors. These results provide an extensive set of ENS biomarkers, demonstrate a role for SNARE proteins in ENS development and highlight additional candidate genes that could modify Hirschsprung's disease penetrance.
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CITATIONS (59)
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