Islet 1 is expressed in distinct cardiovascular lineages, including pacemaker and coronary vascular cells

0301 basic medicine Green Fluorescent Proteins LIM-Homeodomain Proteins Mice 03 medical and health sciences Lineage Biological Clocks Animals Cell Lineage Molecular Biology Homeodomain Proteins 0303 health sciences Myocardium Gene Expression Regulation, Developmental Heart Cell Biology Isl1 Coronary Vessels Cardiac progenitor Pacemaker Tamoxifen Microscopy, Fluorescence Coronary smooth muscle Inducible Cre Developmental Biology Transcription Factors
DOI: 10.1016/j.ydbio.2006.12.048 Publication Date: 2006-12-30T12:07:18Z
ABSTRACT
Islet1 (Isl1) is a LIM homedomain protein that plays a pivotal role in cardiac progenitors of the second heart field. Here, lineage studies with an inducible isl1-cre demonstrated that most Isl1 progenitors have migrated into the heart by E9. Although Isl1 expression is downregulated in most cardiac progenitors as they differentiate, analysis of an isl1-nlacZ mouse and coimmunostaining for Isl1 and lineage markers demonstrated that Isl1 is expressed in distinct subdomains of the heart, and in diverse cardiovascular lineages. Isl1 expression was observed in myocardial lineages of the distal outflow tract, atrial septum, and in sinoatrial and atrioventricular node. The myocardialized septum of the outflow tract was found to derive from Isl1 expressing cells. Isl1 expressing cells also contribute to endothelial and vascular smooth muscle lineages including smooth muscle of the coronary vessels. Our data indicate that Isl1 is a specific marker for a subset of pacemaker cells at developmental stages examined, and suggest genetic heterogeneity within the central conduction system and coronary smooth muscle. Our studies suggest a role for Isl1 in these distinct domains of expression within the heart.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (36)
CITATIONS (281)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....