The Xenopus ORFeome: A resource that enables functional genomics

• DOI: 10.1016/j.ydbio.2015.09.004
• Publication Date: 2015-10-01T06:33:02Z
• Authors: Ian M. Grant; Dawit Balcha; Tong Hao; Yun Shen; Prasad Trivedi; Ilya Patrushev; Joshua D. Fortriede; John B. Karpinka; Limin Liu; Aaron M. Zorn; P. Todd Stukenberg; David E. Hill; Michael J. Gilchrist
• Keywords: Functional Genomics

Functional characterisation of proteins and large-scale, systems-level studies are enabled by extensive sets cloned open reading frames (ORFs) in an easily-accessible format that enables many different applications. Here we report the release first stage Xenopus ORFeome, which contains 8673 ORFs from Gene Collection (XGC) for laevis, into a Gateway® donor vector enabling rapid in-frame transfer to expression vectors. This resource represents estimated 7871 unique genes, approximately 40% non-redundant X. laevis gene complement, includes 2724 genes where human ortholog has association with disease. Transfer Gateway system was validated 5' 3' end sequencing entire collection protein set test clones. In parallel process, underlying ORF predictions original XGC were re-analysed verify quality full-length status, identifying those likely exhibit truncations when translated. These data integrated Xenbase, community database, associates genomic, expression, function disease model metadata each ORF, end-users search ORFeome clones links commercial distributors collection. When coupled experimental advantages eggs embryos, valuable functional genomics modelling.