Establishment and characterization of a novel conditionally immortalized human parietal epithelial cell line

• DOI: 10.1016/j.yexcr.2021.112712
• Publication Date: 2021-06-25T16:29:09Z
• Authors: Laura Miesen; Roy Wetzels; Jennifer Eymael; Fieke Mooren; Valerie Villacort...; Bartholomeus T. v...; Martijn van den B...; Thea J.A.M. van d...; Lambertus P.W.J. ...; Jack F.M. Wetzels; Michiel F. Schreuder; Johan van der Vlag; Jitske Jansen; Bart Smeets
• Keywords: Glomerulosclerosis

Parietal epithelial cells (PECs) are in the kidney, surrounding Bowman's space. When activated, PECs increase cell volume, proliferate, migrate to glomerular tuft and excrete extracellular matrix. Activated crucially involved formation of sclerotic lesions, seen focal segmental glomerulosclerosis (FSGS). In FSGS, a number glomeruli show lesions. Further disease progression will lead increasing gradual destruction affected glomeruli. Although involvement FSGS has been acknowledged, little is known about molecular processes driving PEC activation. To get more insights this process, accurate vivo vitro models needed. Here, we describe development characterization novel conditionally immortalized human (ciPEC) line. We demonstrated that ciPECs differentiated when grown under growth-restrictive conditions express important PEC-specific markers, while lacking podocyte endothelial markers. addition, showed PEC-like morphology responded IL-1β treatment. therefore conclude have successfully generated line, which can be used for future studies on role FSGS.