A parasympathetic neurotransmitter induces myoepithelial cell differentiation during salivary gland development

• DOI: 10.1016/j.yexcr.2022.113137
• Publication Date: 2022-04-12T17:00:51Z
• Authors: Yuki Shindo; Hannah M. Nakamura; Junichi Nakai; Minoru Wakamori; Takashi Nakamura
• Keywords: 0301 basic medicine; Neurotransmitter Agents; Organogenesis; Submandibular Gland; Cholinergic Agents; Cell Differentiation; Epithelial Cells; Receptors, Muscarinic; Acetylcholine; Salivary Glands; Rats; 3. Good health; 03 medical and health sciences; Animals

Myoepithelial cells (MECs) are responsible for receiving stimuli from the central nervous system and translating their responses into form of secretion glandular tissue, including salivary glands (SG), sweet glands, mammary glands. SG MECs cause serous saliva by contracting acini/ductal with acetylcholine (Ach) parasympathetic nerves via muscarinic receptors. To response physiological stimulation, epithelial cell-derived supposed to be induced placed adjacent nerve ends in SGs forming a neuro-myoepithelial junction. For function under control, therefore, specific regions gland must mapped epithelium near differentiate order nerve-myoepithelial junction during organogenesis. We hypothesized that is differentiation which neurotransmitter qPCR whole-mount immunohistochemical analysis ex vivo organ culture revealed were found cholinergic receptor 1 carbachol (CCh), an agonist. In addition, CCh stimulated ERK Akt signaling induction MEC rat submandibular cells. These findings indicate action required formation developing SGs. This study proposes novel concept tissue architecture neurofunctional organogenesis