Evaluation of a fluorescence-polarization assay for the diagnosis of bovine brucellosis in México

• DOI: 10.1016/s0167-5877(99)00004-5
• Publication Date: 2002-07-25T09:22:47Z
• Authors: A Dajer; E Luna-Martı́nez; D Zapata; S Villegas; E Gutiérrez; G Peña; F Gurrı́a; K Nielsen; D Gall
• Keywords: Lipopolysaccharides; Brucellosis, Bovine; Reference Values; Fluorescence Polarization Immunoassay; 0402 animal and dairy science; Animals; Brucella abortus; Cattle Diseases; Cattle; Serologic Tests; 04 agricultural and veterinary sciences; Mexico

A homogeneous fluorescence-polarization assay (FPA) was used for the serological diagnosis of bovine brucellosis in México. The assay uses O-polysaccharide prepared from Brucella abortus lipoplysaccharide (20-30 kDa) conjugated with fluorescein isothiocyanate as a tracer. To measure the fluorescence polarization, a FPM-1 fluorescence-polarization analyzer was used with the procedure described by Nielsen et al. (1996b). A cut-off value of 90 millipolarization (mP) units was used for testing 560 bovine sera from different areas of México. (305 positive sera and 255 negative sera according to the complement fixation test; CFT.) Some were tested with the Rose Bengal plate (RB) test (n = 490) and some with the rivanol-agglutination (RIV) test (n = 190). Sensitivities were 98.3%, 99.3% and 99.0%, and specificities were 68.8%, 55.4% and 96.9%, respectively, for RB, RIV and FPA. The FPA gave a kappa coefficient of agreement with respect to CFT of 0.96, while RB and RIV (relative to the CFT) gave coefficients of 0.70 and 0.61, respectively. Finally, ROC analysis suggested a cut-off value which agreed with the one recommended in the test procedure. We concluded that FPA is a suitable test to be used instead of the CFT in Mexican conditions.